Effect of age, sex, and body size on the blood biochemistry and physiological constants of dogs from 4 wk. to > 52 wk. of age

Abstract Background Blood biochemistry and reference intervals help to differentiate between healthy ill dogs as well provide information for the prognosis, evaluation, monitoring; however, these are often obtained from adult animals. It is essential understand that puppies adults physiologically different, which justifies need obtain age-specific biochemical intervals. The aim of this research was assess potential effect age, sex, body size, their interaction on routine analytes physiological constants (body temperature, heart rate, respiratory rate). To carry out research, we selected 197 both sexes different sizes (small, medium large) classified by age: group I (4–8 wk), II (9–24 III (25–52 IV (> 52 wk). analysis included measurement enzymatic activity aspartate aminotransferase (AST), alanine (ALT), lactate dehydrogenase (LDH), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), concentrations cholesterol, triglycerides, total proteins, albumin, globulins, glucose, urea, creatinine. Statistical analyses used variance (ANOVA) a general linear model (GLM), allows comparison multiple factors at two or more levels ( p < 0.05). Results results study showed ALT, protein, globulin, creatinine, temperature were lower in than 0.05), while ALP, LDH, glucose concentration, rate higher. Whereas size did not show significant > Conclusions Some components influenced age. For reason, manuscript contributes with additional data clinical interpretation blood puppies.